Angiotensin II Induces p67 mRNA Expression and NADPH Oxidase Superoxide Generation in Rabbit Aortic Adventitial Fibroblasts

Superoxide radical (O2 ) is ubiquitously critical to the bioactivity of endothelial nitric oxide. In angiotensindependent hypertension, vascular O2 2 levels rise and impede endothelium/nitric oxide–dependent vascular relaxation. We have reported that the major O2 2 source in the rabbit aorta is adventitial fibroblast phagocyte-like NADPH oxidase and shown that angiotensin (Ang) II treatment of adventitial fibroblasts causes a concentration-dependent increase in particulate NADPH-dependent O2 . From cultured rabbit aortic adventitial fibroblasts treated or not treated with Ang II, we prepared particulate fractions and measured lucigenin-enhanced chemiluminescence. Because [Sar,Thr]-Ang II, a generalized antagonist of Ang II and plausible inhibitor of the conversion of Ang II, reversed Ang II (10 nmol/L)–induced NADHand NADPH-dependent O2 2 to basal levels, we tested the effect of the inhibitor of aminopeptidase N, amastatin (10 mmol/L), and found no effect on Ang II–stimulated O2 . Ang(1-7), Ang III, and Ang IV also were not effective in stimulating O2 2 levels at concentrations similar to those of Ang II. Kinetic analysis showed a rise in NADPH oxidase O2 2 production in response to Ang II, which peaks at 3 hours and returns to basal levels by 16 hours. p67, a cytosolic factor, appears to be affected at both the level of transcription and protein synthesis because actinomycin and cycloheximide individually inhibited the observed effect. A partial sequence of p67 was recovered by reverse transcriptase from mRNA harvested from cultured rabbit aortic adventitial fibroblasts. Furthermore, the p67 mRNA transcript in aortic fibroblasts is induced by Ang II before the peak of NADPH oxidase by Northern analysis and ribonuclease protection assays. These data suggest that Ang II stimulates NAD(P)H oxidase O2 2 generation in fibroblasts of aortic adventitia via transcriptional activation of p67. These data also provide preliminary evidence for the regulation of factors of the NADPH oxidase and potentially provide a novel means by which to abrogate the development of O2 -dependent hypertension. (Hypertension. 1998;32:331-337.)